Coding

Part:BBa_K1702000:Design

Designed by: Anthony Roulier, Chauncy Hinshaw, Krista Henderson   Group: iGEM15_CSU_Fort_Collins   (2015-09-17)


Overexpression of E. coli fadD under control of lac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This plasmid is designed to allow for induced transcription with lactose or IPTG.


Source

This fadD is endogenous to E. coli and was extracted by genomic PCR from DH5alpha E. coli cells.

References